①不同样本的多酚氧化酶最佳反应温度略有差别,反应温度可在25-37℃之间进行调整;
②若A测定大于1.5或ΔA大于1.0,建议将粗酶液适当稀释后再进行测定;若ΔA小于0.02,建议适当延长酶促反应时间或适当增加样本量后再进行测定,计算时相应修改;
[1] Cui Y, Wang Z, Zhang G, et al. Transmission of nanoplastics from Culex quinquefasciatus to Pardosa pseudoannulata and its impact on predators[J]. Science of The Total Environment, 2022, 820: 153331.(IF 10.753)
[2] Liu Y, Yan Y, Ma L, et al. Physiological and metabolomics analyses reveal the resistance response mechanism to tea aphid infestation in new shoots of tea plant (Camellia sinensis)[J]. Plant Stress, 2024: 100545. (IF 6.8)
[3] Ouyang W, Ning J, Zhu X, et al. UPLC-ESI-MS/MS analysis revealed the dynamic changes and conversion mechanism of non-volatile metabolites during green tea fixation[J]. LWT, 2024: 116010. (IF 6.0)
[4] Li G, Mo Y, Lv J, et al. Unraveling verticillium wilt resistance: insight from the integration of transcriptome and metabolome in wild eggplant[J]. Frontiers in Plant Science, 2024, 15: 1378748. (IF 5.6)
[5] Li X, Xiu D, Huang J, et al. Nutshell Physicochemical Characteristics of Different Hazel Cultivars and Their Defensive Activity toward Curculio nucum (Coleoptera: Curculionidae)[J]. Forests, 2023, 14(2): 319.(IF 2.9)