Hydrogen Peroxide (H₂O₂) Content Assay Kit
过氧化氢含量检测试剂盒
货号:AKAO009M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Hydrogen Peroxide (H₂O₂) Content Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:5 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    丙酮(CH3COCH3,MW=58.08,CAS: 67-64-1);
    浓盐酸(HCl,MW=36.46,CAS: 7647-01-0)
Detection Principle
H2O2与硫酸钛反应生成黄色过氧化-钛复合物沉淀,产物在415 nm处具有特征吸收峰,通过吸光值变化即可定量检测H2O2的含量。
  • 检测方法: 硫酸钛法
  • 检测波长: 415 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Hydrogen Peroxide
  • 参考标准: y=0.3759x-0.0018 (R2=0.9999)
  • 标准线性范围: 0.25-2.5 µmol/mL
  • 检测限: 0.01 µmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①丙酮易挥发,4℃预冷后再使用,研磨时必须在冰上进行;

②试剂的挥发性均较高,建议在通风厨中进行操作,并做好防护措施;

③若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本使用丙酮适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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