Total Antioxidant Capacity (T-AOC) Assay Kit
总抗氧化能力检测试剂盒
货号:AKAO012C
规格: 70T/50S
检测设备:可见分光光度计
可检测样本数:50 Samples
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Product Information
Total Antioxidant Capacity (T-AOC) Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:3 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    浓硫酸(H2SO4,MW=98.078,CAS: 7664-93-9)
Detection Principle
本产品采用FRAP(Ferric Reducing Ability of Plasma)法测定总抗氧化能力,其原理是酸性条件下抗氧化物可以将Fe3+-TPTZ还原为蓝色的络合物Fe2+-TPTZ,产物在593 nm处具有特征吸收峰,通过吸光值的变化可以反映待测样品的还原能力,即样品的总抗氧化能力。
  • 检测方法: FRAP法
  • 检测波长: 593 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: FeSO4
  • 参考标准: y=19.942x+0.0032 (R2=0.9999)
  • 标准线性范围: 0.0015625-0.05 μmol/mL
  • 检测限: 0.0006 μmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①试剂二具有刺激性应避免接触,注意采取适当的防护措施,请穿实验服并戴乳胶手套操作;

②尽量避免使用在酸性条件下呈蓝色或接近蓝色的样本,否则对本试剂盒的检测结果产生干扰;

③样品中不宜添加Tween和NP-40等去垢剂和DTT、巯基乙醇等影响氧化还原反应的还原剂;

④若测定吸光值超出标准线性吸光值范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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[3] Zhang Y, Liu Y, Liu X, et al. Exercise and metformin intervention prevents lipotoxicity-induced hepatocyte apoptosis by alleviating oxidative and ER stress and activating the AMPK/Nrf2/HO-1 signaling pathway in db/db mice[J]. Oxidative Medicine and Cellular Longevity, 2022, 2022.(IF 7.31)

[4] Wang Z, Jiang Q, Li P, et al. The Water Extract of Ampelopsis grossedentata Alleviates Oxidative Stress and Intestinal Inflammation[J]. Antioxidants, 2023, 12(3): 547.(IF 7)

[5] Li K, Xu R, Cao J, et al. Nordihydroguaiaretic acid (NDGA) maintains the postharvest quality of fresh daylily flower buds (Hemerocallis citrina) by enhancing antioxidant metabolism and regulating phytohormone equilibrium[J]. Postharvest Biology and Technology, 2023, 200: 112326.(IF 7)

[6] Chen Y, Xu Y, Li D, et al. Exogenous azacytidine alleviates peel browning of postharvest bananas (Musa acuminata) showing an important role of DNA methylation[J]. Postharvest Biology and Technology, 2023, 206: 112554.(IF 7)

[7] Xiang X, Wang H, Zhou W, et al. Glutathione Protects against Paraquat-Induced Oxidative Stress by Regulating Intestinal Barrier, Antioxidant Capacity, and CAR Signaling Pathway in Weaned Piglets[J]. Nutrients, 2023, 15(1): 198.(IF 6.706)

[8] Chen J, Li Y, Li F, et al. Physiological, metabolome, and transcriptome analysis revealed the effect of ethyl 3-amino-3-thioxopropanoate on the browning of fresh-cut banana during storage[J]. Postharvest Biology and Technology, 2024, 218: 113176. (IF 6.4)

[9] Tang W, Xiang X, Wang H, et al. Zinc lactate alleviates oxidative stress by modulating crosstalk between constitutive androstane receptor signaling pathway and gut microbiota profile in weaned piglets[J]. Animal Nutrition, 2023.(IF 6.3)

[10] Wang M, Jiang Y, Wang S, et al. Yak milk protects against alcohol-induced liver injury in rats[J]. Food & Function, 2023, 14(21): 9857-9871.(IF 6.1)

[11] Tang J, Zhu Z, He H, et al. Bacterioferritin: a key iron storage modulator that affects strain growth and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona[J]. Microbial Cell Factories, 2021, 20(1): 1-16.(IF 5.328)

[12] Yang Z, Wang F, Yin Y, et al. Dietary Litsea cubeba essential oil supplementation improves growth performance and intestinal health of weaned piglets[J]. Animal Nutrition, 2023, 13: 9-18.(IF 5.285)

[13] Yu J, Jiang W, Wang S, et al. Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora[J]. Journal of Functional Foods, 2022, 97: 105255.(IF 5.223)

[14] Lin Z, Li Y, Wang M, et al. Protective effects of yeast extract against alcohol-induced liver injury in rats[J]. Frontiers in Microbiology, 2023, 14.(IF 5.2)

[15] Qian Y, Guo Y, Li Y, et al. The antihypertensive effect of Alizarin is achieved by activating VEGFR2/eNOS pathway, attenuating oxidative stress-induced mitochondrial damage and premature senescence[J]. Life Sciences, 2024: 122862. (IF 5.2)

[16] Zhang G Q, Liu W, Shi Y H, et al. Intrinsic peroxidase activity of carbon dots derived from expired drugs and its application in automatic detection of total antioxidant capacity[J]. Microchemical Journal, 2023: 109720.(IF 4.8)

[17] Tang M, Zhao J, Wu Y, et al. Improving Gut Functions and Egg Nutrition with Stevia Residue in Laying Hens: METABOLISM AND NUTRITION[J]. Poultry Science, 2023: 103324.(IF 4.4)

[18] Zhang H, Xiang X, Wang C, et al. Different effects of acute and chronic oxidative stress on the intestinal flora and gut-liver axis in weaned piglets[J]. Frontiers in Microbiology, 2024, 15: 1414486. (IF 4.0)

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