①试剂二具有刺激性应避免接触,注意采取适当的防护措施,请穿实验服并戴乳胶手套操作;
②尽量避免使用在酸性条件下呈蓝色或接近蓝色的样本,否则对本试剂盒的检测结果产生干扰;
③样品中不宜添加Tween和NP-40等去垢剂和DTT、巯基乙醇等影响氧化还原反应的还原剂;
④若测定吸光值超出标准线性吸光值范围:高于最高值建议将待测样本适当稀释后再进行测定,低于最低值建议适当增加样本量后再进行测定,计算时相应修改;
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