①不同样本羟自由基清除能力可能相差较大,若需要比较不同样本的羟自由基清除能力,建议对于同一批植物样本加入等量的样本,液体样本加入相同体积,提取物或者药物配制为相同浓度;将样本根据预实验结果进行适当调整,比较同样浓度(相同稀释倍数)的清除率大小;
②若测定样本较多时,可按试剂一:试剂二:试剂三=1:2:2的体积比配制为检测工作液,根据使用量现用现配;
③若A测定大于0.5,建议将待测样本使用提取液适当稀释后再进行测定;若A测定小于0.05,建议适当增加样本质量或液体样本体积重新提取后再进行测定,计算时相应修改;
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