①样品提取过程建议在冰上完成操作,且提取后应当天完成测定;
②若待测样本DPPH自由基清除率(DS%)大于90%,建议将待测样本使用提取液稀释后再进行测定;若待测样本DPPH自由基清除率(DS%)小于5%,建议适当增加烘干样本质量或液体样本体积重新提取后再进行测定,计算时相应修改;
③不同样本DPPH自由基清除能力可能相差较大,若需要比较不同样本的DPPH自由基清除能力,建议对于同一批植物样本加入等量的样本,液体样本加入相同体积,提取物或者药物配制为相同浓度;将样本根据预实验结果进行适当调整,比较同样浓度(相同稀释倍数)的清除率大小;
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