DPPH Free Radical Scavenging Ability Assay Kit
DPPH自由基清除能力检测试剂盒
货号:AKAO020C
规格: 70T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
DPPH Free Radical Scavenging Ability Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:4 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
DPPH自由基具有单电子,其有机溶液呈紫色,在515 nm处具有特征吸收峰,当有抗氧化剂存在时,DPPH自由基接受一个电子或氢原子,形成稳定的DPPH-H化合物,使溶液从紫色变为黄色,变色程度与其自由基清除活性呈定量关系,通过吸光度的变化即可表征DPPH自由基清除能力,并提供Trolox作为阳性对照量化抗氧化物质的清除能力。
  • 检测方法: DPPH法
  • 检测波长: 515 nm
  • 信号响应: 递减型
Refreence Information
  • 标准物质: Trolox
  • 参考标准: y=117.92x-0.4933(R2=0.9995)
  • 标准线性范围: 0.05-0.8 mmol/L
  • 检测限: -
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①样品提取过程建议在冰上完成操作,且提取后应当天完成测定;

②若待测样本DPPH自由基清除率(DS%)大于90%,建议将待测样本使用提取液稀释后再进行测定;若待测样本DPPH自由基清除率(DS%)小于5%,建议适当增加烘干样本质量或液体样本体积重新提取后再进行测定,计算时相应修改;

③不同样本DPPH自由基清除能力可能相差较大,若需要比较不同样本的DPPH自由基清除能力,建议对于同一批植物样本加入等量的样本,液体样本加入相同体积,提取物或者药物配制为相同浓度;将样本根据预实验结果进行适当调整,比较同样浓度(相同稀释倍数)的清除率大小;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Liu L, Cui H, Huang Y, et al. Enzyme-mediated reactions of phenolic pollutants and endogenous metabolites as an overlooked metabolic disruption pathway[J]. Environmental Science & Technology, 2022, 56(6): 3634-3644.(IF 11.357)

[2] Lin X, Chang M, Cao M, et al. Trimetallic-doped carbon nitride achieves chondroitin sulfate degradation via a free radical degradation strategy[J]. Carbohydrate Polymers, 2024: 122616. (IF 10.7)

[3] Li K, Xu R, Cao J, et al. Nordihydroguaiaretic acid (NDGA) maintains the postharvest quality of fresh daylily flower buds (Hemerocallis citrina) by enhancing antioxidant metabolism and regulating phytohormone equilibrium[J]. Postharvest Biology and Technology, 2023, 200: 112326.(IF 7)

[4] Chen Y, Xu Y, Li D, et al. Exogenous azacytidine alleviates peel browning of postharvest bananas (Musa acuminata) showing an important role of DNA methylation[J]. Postharvest Biology and Technology, 2023, 206: 112554.(IF 7)

[5] Chen J, Li Y, Li F, et al. Physiological, metabolome, and transcriptome analysis revealed the effect of ethyl 3-amino-3-thioxopropanoate on the browning of fresh-cut banana during storage[J]. Postharvest Biology and Technology, 2024, 218: 113176. (IF 6.4)

[6] Peng C, Deng L, Tan H, et al. Transcriptome and metabolome analysis of preharvest internal browning in Nane plum fruit caused by high temperatures[J]. Horticultural Plant Journal, 2024. (IF 5.7)

[7] Wen H, Kuang Y, Lian X, et al. Physicochemical Characterization, Antioxidant and Anticancer Activity Evaluation of an Acidic Polysaccharide from Alpinia officinarum Hance[J]. Molecules, 2024, 29(8): 1810. (IF 4.6)

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