Biuret Method Protein Content Assay Kit
双缩脲法蛋白含量检测试剂盒
货号:AKPR001M
规格: 110T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Biuret Method Protein Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    蒸馏水、生理盐水或不含蛋白的PBS
Detection Principle
蛋白质能够在强碱性溶液中与双缩脲试剂形成紫色络合物,产物在540 nm处具有特征吸收峰,通过吸光值的变化即可定量检测蛋白含量。
  • 检测方法: 双缩脲法
  • 检测波长: 540 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: BSA
  • 参考标准: y=0.0213x-0.002 (R2=0.9995)
  • 标准线性范围: 0.5-10 mg/mL
  • 检测限: 0.5 mg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①待测样本蛋白浓度须在0.5-10 mg/mL范围内,正式测定应取1-2个差异样本进行预实验,确保蛋白浓度在此范围内,低于0.5 mg/mL则不能使用此法测定,高于10 mg/mL或吸光值大于0.2应使用Extract Solution适当稀释后再进行测定,计算时相应修改。
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Du P, Song J, Qiu H, et al. Polyphenols Extracted from Shanxi-Aged Vinegar Inhibit Inflammation in LPS-Induced RAW264. 7 Macrophages and ICR Mice via the Suppression of MAPK/NF-κB Pathway Activation[J]. Molecules, 2021, 26(9): 2745.(IF 4.412)

[2] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4)

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