①样品处理过程应在冰上迅速完成,提取后待测样本应置于冰上保存且建议当天完成测定;
②准确在30 s和150 s处完成吸光值测定,以确保实验结果的准确性和重复性;若同时测定多个样本时,应使用多道移液器且分批进行测定,以确保组间反应时间一致;
③试剂一中含有蛋白质沉淀剂,待测样本不能用于蛋白浓度测定,需另取组织使用1×PBS或生理盐水按照与待测样本相同的样本浓度重新提取后,再进行蛋白含量测定;
④若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本使用试剂一适当稀释后再进行测定;低于最低值建议制备更高浓度样本后再进行测定,计算时相应修改;
⑤试剂二和试剂六试剂量较小,运输过程中可能会使试剂残留在管壁或管帽上,使用前建议先离心收集(可参考使用1000 g常温离心1-2 min),再使用移液器缓慢吹打混匀后使用;
⑥试剂五配制后有效期较短,为便于试验安排,附赠1瓶作为备用;
⑦试剂二具有刺激性气味,建议使用时在通风橱中操作且做好防护措施;
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