Oxidized Glutathione (GSSG) Content Assay Kit
氧化型谷胱甘肽含量检测试剂盒
货号:AKPR009M
规格: 120T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Oxidized Glutathione (GSSG) Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
通过GSH掩蔽剂去除样本中原有的GSH,并在谷胱甘肽还原酶作用下将GSSG还原为GSH,GSH能够与5,5’-二硫代-双-(2-硝基苯甲酸)(DTNB)反应生成2-硝基-5-巯基苯甲酸(TNB),产物在412 nm处具有特征吸收峰,通过吸光值变化即可定量检测氧化型谷胱甘肽的含量。
  • 检测方法: DTNB法
  • 检测波长: 412 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Oxidized Glutathione
  • 参考标准: y=0.0157x+0.0005 (R2=0.9993)
  • 标准线性范围: 5-60 µg/mL
  • 检测限: 5 µg/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①样品处理过程应在冰上迅速完成,提取后待测样本应置于冰上保存且建议当天完成测定;

②准确在30 s和150 s处完成吸光值测定,以确保实验结果的准确性和重复性;若同时测定多个样本时,应使用多道移液器且分批进行测定,以确保组间反应时间一致;

③试剂一中含有蛋白质沉淀剂,待测样本不能用于蛋白浓度测定,需另取组织使用1×PBS或生理盐水按照与待测样本相同的样本浓度重新提取后,再进行蛋白含量测定;

④若测定吸光值超出标准吸光值线性范围:高于最高值建议将待测样本使用试剂一适当稀释后再进行测定;低于最低值建议制备更高浓度样本后再进行测定,计算时相应修改;

⑤试剂二和试剂六试剂量较小,运输过程中可能会使试剂残留在管壁或管帽上,使用前建议先离心收集(可参考使用1000 g常温离心1-2 min),再使用移液器缓慢吹打混匀后使用;

⑥试剂五配制后有效期较短,为便于试验安排,附赠1瓶作为备用;

⑦试剂二具有刺激性气味,建议使用时在通风橱中操作且做好防护措施;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Wang Y, Lu C, Guo S, et al. Leafhopper salivary vitellogenin mediates virus transmission to plant phloem[J]. Nature Communications, 2024, 15(1): 3.(IF 16.6)

[2] Wang X, Wu H, Yu Z, et al. Plant viruses exploit insect salivary GAPDH to modulate plant defenses[J]. Nature Communications, 2024, 15(1): 6918. (IF 14.7)

[3] Sun W, Wu G, Xu H, et al. Malate-mediated CqMADS68 enhances aluminum tolerance in quinoa seedlings through interaction with CqSTOP6, CqALMT6 and CqWRKY88[J]. Journal of Hazardous Materials, 2022, 439: 129630.(IF 14.224)

[4] Li C, Cui Z, Deng S, et al. Programmed Cell Death Protein 2-like Promotes Inflammation and Oxidative Stress in Vascular Endothelial Cells[J]. ACS Pharmacology & Translational Science, 2023, 6(10): 1453-1470.(IF 6)

[5] Wang H, Wang Z, Gao Y, et al. STZ-induced diabetes exacerbates neurons ferroptosis after ischemic stroke by upregulating LCN2 in neutrophils[J]. Experimental Neurology, 2024: 114797. (IF 5.3)

[6] Tao D, Wang Y, Liu J, et al. Mechanism of CuSO4 cytotoxicity in goat erythrocytes after high-level in vitro exposure to isotonic media[J]. Ecotoxicology and Environmental Safety, 2021, 208: 111730.(IF 4.872)

[7] Zhang Q, Lin J, Yan J, et al. Enhanced Heat Resistance in Morchella eximia by Atmospheric and Room Temperature Plasma[J]. Microorganisms, 2024, 12(3): 518. (IF 4.5)

[8] Xing S, Wang M, Zhang Z, et al. Sulfur dioxide enhances postharvest grape resistance to Botrytis cinerea by promoting glutathione level[J]. Scientia Horticulturae, 2024, 334: 113295. (IF 4.3)

[9] Zhou H, Wan S, Luo Y, et al. Hepatitis B virus X protein induces ALDH2 ubiquitin-dependent degradation to enhance alcoholic steatohepatitis[J]. Gastroenterology Report, 2023, 11: goad006.(IF 3.6)

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