Glutathione Peroxidase (GPX) Activity Assay Kit
谷胱甘肽过氧化物酶活性检测试剂盒
货号:AKPR014C
规格: 60T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
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Product Information
Glutathione Peroxidase (GPX) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:4 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
谷胱甘肽过氧化物酶可催化H2O2氧化GSH生成GSSG,GSH和5,5’-二硫代-双-(2-硝基苯甲酸)反应生成黄色的2-硝基-5-巯基苯甲酸,产物在412 nm处有特征吸收峰,通过吸光值下降速率即可表征谷胱甘肽过氧化物酶活性。
  • 检测方法: DTNB法
  • 检测波长: 412 nm
  • 信号响应: 递减型
Notices

①若测定吸光值大于1.0,建议将粗酶液使用提取液适当稀释后再进行测定,计算时相应修改;

②显色完成后应立即测定吸光值,以免检测时间过长影响显色造成结果误差。

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Cui Y, Wang Z, Zhang G, et al. Transmission of nanoplastics from Culex quinquefasciatus to Pardosa pseudoannulata and its impact on predators[J]. Science of The Total Environment, 2022, 820: 153331.(IF 10.753)

[2] Wu Y T, Zhong L S, Huang C, et al. β-Caryophyllene Acts as a Ferroptosis Inhibitor to Ameliorate Experimental Colitis[J]. International Journal of Molecular Sciences, 2022, 23(24): 16055.(IF 6.208)

[3] Wang M, Jiang Y, Wang S, et al. Yak milk protects against alcohol-induced liver injury in rats[J]. Food & Function, 2023, 14(21): 9857-9871.(IF 6.1)

[4] Yang Z, Wang F, Yin Y, et al. Dietary Litsea cubeba essential oil supplementation improves growth performance and intestinal health of weaned piglets[J]. Animal Nutrition, 2023, 13: 9-18.(IF 5.285)

[5] Lin Z, Li Y, Wang M, et al. Protective effects of yeast extract against alcohol-induced liver injury in rats[J]. Frontiers in Microbiology, 2023, 14.(IF 5.2)

[6] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4)

[7] Zhang B, Li Y, Liu N, et al. AP39, a novel mitochondria-targeted hydrogen sulfide donor ameliorates doxorubicin-induced cardiotoxicity by regulating the AMPK/UCP2 pathway[J]. Plos one, 2024, 19(4): e0300261. (IF 3.7)

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