①实验过程,所有试剂都要需要预冷或者即融,保证操作过程中的低温环境;
②PSMF具有毒性且在水溶液中会快速降解,应现用现加,注意做好防护措施;
③Lysis Buffer中含有Protease Inhibitors,提取后植物蛋白不能用于蛋白激酶的研究
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