①试剂三和试剂四配制后有效期较短,为便于试验安排,各附赠一瓶作为备用,每瓶均可满足至少25个样本的测定;
②试剂五配制后未使用完试剂应置于4℃保存,严禁-20℃保存,使用过程中试剂五应用液需置于冰上放置;
③2 µmol/mL NAD+标准液和2 µmol/mL NADH标准液配制后4℃可保存1周,严禁-20℃保存;
④实验过程中吸取上清液时应避免吸入沉淀物而影响结果的准确性;
⑤反应时间对实验结果有较大影响建议精确控制,测定样本较多时建议分批进行测定,以避免组内反应时间不一致对结果造成影响;
⑥反应体系中试剂不能按比例配制为混合液使用,必须按反应体系顺序依次加入;
⑦若A测定大于1.0,建议将待测样本适当稀释后再进行测定,计算时相应修改;
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