Coenzyme Ⅰ NAD (H) Content Assay Kit
辅酶Ⅰ NAD(H)含量检测试剂盒
货号:AKCO001M
规格: 240T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Coenzyme Ⅰ NAD (H) Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:6 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5)
Detection Principle
通过酸性和碱性提取液分别提取样品中NAD+和NADH,NADH可通过PMS的递氢作用,还原氧化型噻唑蓝(MTT)生成甲瓒,产物在570 nm处具有特征吸收峰;NAD+可被乙醇脱氢酶还原为NADH,进一步采用MTT还原法生成甲瓒,通过吸光值变化即可定量检测辅酶Ⅰ NAD (H) 的含量。
  • 检测方法: MTT还原法
  • 检测波长: 570 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: NAD+ & NADH
  • 参考标准: y=0.1262x-0.0029 (R2=0.9995)
  • 标准线性范围: 0.25-5.0 nmol/mL
  • 检测限: 0.1 nmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①试剂三和试剂四配制后有效期较短,为便于试验安排,各附赠一瓶作为备用,每瓶均可满足至少50个样本的测定;

②试剂五配制后未使用完试剂应置于4℃保存,严禁-20℃保存,使用过程中试剂五应用液需置于冰上放置;

③2 µmol/mL NAD+标准液和2 µmol/mL NADH标准液配制后4℃可保存1周,严禁-20℃保存;

④实验过程中吸取上清液时应避免吸入沉淀物而影响结果的准确性;

⑤反应时间对实验结果有较大影响建议精确控制,测定样本较多时建议分批进行测定,以避免组内反应时间不一致对结果造成影响;

⑥反应体系中试剂不能按比例配制为混合液使用,必须按反应体系顺序依次加入;

⑦若A测定大于1.0,建议将待测样本适当稀释后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Li S, Zhao Y, Wu S, et al. Regulation of species metabolism in synthetic community systems by environmental pH oscillations[J]. Nature Communications, 2023, 14(1): 7507.(IF 16.6)

[2] Cui Z, Zhong Y, Sun Z, et al. Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica[J]. Nature Communications, 2023, 14(1): 8480.(IF 16.6)

[3] Yang Y, Liu C, Zhao W, et al. Anaerobic propionic acid production via succinate pathway at extremely low pH[J]. Chemical Engineering Journal, 2024: 150190. (IF 15.1)

[4] Lin S, Wang T, Tao Z, et al. Shewanella oneidensis-based artificial conductive micro-niche for hydrogen augmentation[J]. Chemical Engineering Journal, 2024: 150850. (IF 15.1)

[5] Chen T T, Zhao P, Wang Y, et al. The plastid‐localized lipoamide dehydrogenase 1 is crucial for redox homeostasis, tolerance to arsenic stress and fatty acid biosynthesis in rice[J]. New Phytologist, 2024. (IF 9.4)

[6] Liu G, Yi Z, Li J, et al. Detoxification with resin promotes the shift from acidogenesis to solventogenesis and prevents acid crash during butanol fermentation from wheat straw[J]. Biomass Conversion and Biorefinery, 2023: 1-10.(IF 4)

[7] Zhang S Y, Gao H, Askar A, et al. Steroid hormone 20‐hydroxyecdysone disturbs fat body lipid metabolism and negatively regulates gluconeogenesis in Hyphantria cunea larvae[J]. Insect Science, 2023, 30(3): 771-788.(IF 3.605)

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