①试剂三和试剂四配制后有效期较短,为便于试验安排,各附赠一瓶作为备用,每瓶均可满足至少50个样本的测定;
②试剂五配制后未使用完试剂应置于4℃保存,严禁-20℃保存,使用过程中试剂五应用液需置于冰上放置;
③2 µmol/mL NAD+标准液和2 µmol/mL NADH标准液配制后4℃可保存1周,严禁-20℃保存;
④实验过程中吸取上清液时应避免吸入沉淀物而影响结果的准确性;
⑤反应时间对实验结果有较大影响建议精确控制,测定样本较多时建议分批进行测定,以避免组内反应时间不一致对结果造成影响;
⑥反应体系中试剂不能按比例配制为混合液使用,必须按反应体系顺序依次加入;
⑦若A测定大于1.0,建议将待测样本适当稀释后再进行测定,计算时相应修改;
[1] Li S, Zhao Y, Wu S, et al. Regulation of species metabolism in synthetic community systems by environmental pH oscillations[J]. Nature Communications, 2023, 14(1): 7507.(IF 16.6)
[2] Cui Z, Zhong Y, Sun Z, et al. Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica[J]. Nature Communications, 2023, 14(1): 8480.(IF 16.6)
[3] Yang Y, Liu C, Zhao W, et al. Anaerobic propionic acid production via succinate pathway at extremely low pH[J]. Chemical Engineering Journal, 2024: 150190. (IF 15.1)
[4] Lin S, Wang T, Tao Z, et al. Shewanella oneidensis-based artificial conductive micro-niche for hydrogen augmentation[J]. Chemical Engineering Journal, 2024: 150850. (IF 15.1)
[5] Chen T T, Zhao P, Wang Y, et al. The plastid‐localized lipoamide dehydrogenase 1 is crucial for redox homeostasis, tolerance to arsenic stress and fatty acid biosynthesis in rice[J]. New Phytologist, 2024. (IF 9.4)
[6] Liu G, Yi Z, Li J, et al. Detoxification with resin promotes the shift from acidogenesis to solventogenesis and prevents acid crash during butanol fermentation from wheat straw[J]. Biomass Conversion and Biorefinery, 2023: 1-10.(IF 4)
[7] Zhang S Y, Gao H, Askar A, et al. Steroid hormone 20‐hydroxyecdysone disturbs fat body lipid metabolism and negatively regulates gluconeogenesis in Hyphantria cunea larvae[J]. Insect Science, 2023, 30(3): 771-788.(IF 3.605)