L-Lactate Dehydrogenase (L-LDH) Activity Assay Kit
L-乳酸脱氢酶活性检测试剂盒
货号:AKCO003M
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
L-Lactate Dehydrogenase (L-LDH) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
L-乳酸脱氢酶能够催化NAD+氧化L-乳酸生成丙酮酸,丙酮酸进一步与2,4-二硝基苯肼反应生成丙酮酸二硝基苯腙,在碱性溶液中显棕红色,产物在450 nm处具有特征吸收峰,通过吸光值变化即可表征L-乳酸脱氢酶活性。
  • 检测方法: 2, 4-二硝基苯肼法
  • 检测波长: 450 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: Sodium Pyruvate
  • 参考标准: y=0.3039x-0.0004 (R2=0.9994)
  • 标准线性范围: 0.0625-1.2 μmol/mL
  • 检测限: To Be Updated
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若测定吸光值超出标准吸光值线性范围:高于最高值建议将粗酶液适当稀释后再进行测定;低于最低值建议适当增加酶促反应时间或增加样本量重新制备粗酶液后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

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[4] He Y, Jiang S, Cui Y, et al. Induction of IFIT1/IFIT3 and inhibition of Bcl-2 orchestrate the treatment of myeloma and leukemia via pyroptosis[J]. Cancer Letters, 2024: 216797. (IF 9.7)

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[9] Yu J, Jiang W, Wang S, et al. Microencapsulated hawthorn berry polyphenols alleviate exercise fatigue in mice by regulating AMPK signaling pathway and balancing intestinal microflora[J]. Journal of Functional Foods, 2022, 97: 105255.(IF 5.223)

[10] Lin Z, Li Y, Wang M, et al. Protective effects of yeast extract against alcohol-induced liver injury in rats[J]. Frontiers in Microbiology, 2023, 14.(IF 5.2)

[11] Su J H, Wu G P, Peng X, et al. Neuroprotective Effects of an N-Salicyloyl Tryptamine Derivative against Cerebral Ischemia/Reperfusion Injury[J]. ACS Chemical Neuroscience, 2023.(IF 5)

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[13] Du P, Li N, Wang J, et al. Structural Characterization and Antifatigue Activity of a Novel Exopolysaccharide Isolated from Marasmius androsaceus[J]. Journal of Food Biochemistry, 2023, 2023.(IF 4)

[14] Zhang H, Xiang X, Wang C, et al. Different effects of acute and chronic oxidative stress on the intestinal flora and gut-liver axis in weaned piglets[J]. Frontiers in Microbiology, 2024, 15: 1414486. (IF 4.0)

[15] Tian S, Xu Y, Zhong Y, et al. Exploring the Organic Acid Secretion Pathway and Potassium Solubilization Ability of Pantoea vagans ZHS-1 for Enhanced Rice Growth[J]. Plants, 2024, 13(14). (IF 4.0)

[16] Liu J, Zuo X, Bi J, et al. Palliative Effect of Combined Application of Zinc and Selenium on Reproductive Injury Induced by Tripterygium Glycosides in Male Rats[J]. Biological Trace Element Research, 2024: 1-13.(IF 3.9)

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