Acetaldehyde Dehydrogenase (ALDH) Activity Assay Kit
乙醛脱氢酶活性检测试剂盒
货号:AKCO004U
规格: 60T/50S
检测设备:紫外分光光度计
可检测样本数:50 Samples
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Product Information
Acetaldehyde Dehydrogenase (ALDH) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
乙醛脱氢酶能够催化乙醛和NAD+转化为乙酸和NADH,NADH在340 nm处具有特征吸收峰,通过吸光值变化即可表征乙醛脱氢酶的活性。
  • 检测方法: NADH速率法
  • 检测波长: 340 nm
  • 信号响应: 递增型
Notices

①若吸光值大于3.0,请确认比色皿是否为石英比色皿(Q);

②若A2测定大于1.5或∆A大于1.0,建议将粗酶液使用蒸馏水适当稀释后再进行测定;若∆A小于0.02,建议适当增加样本量或延长酶促反应时间(延长5-10 min)后再进行测定,计算时相应修改;

③准确在30 s和90 s处完成吸光值测定,以保证实验结果的准确性和重复性;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Shan G, Bian Y, Yao G, et al. Targeting ALDH2 to Augment Platinum-Based Chemosensitivity through Ferroptosis in Lung Adenocarcinoma[J]. Free Radical Biology and Medicine, 2024. (IF 7.1)

[2] Chai Y, Zi C, Wei G, et al. Molecular mechanisms of high aldehyde dehydrogenase production for improved beany flavour regulated by the LuxS/AI-2 quorum sensing system in Limosilactobacillus fermentum A119[J]. LWT, 2024: 116674. (IF 6.0)

[3] Xu H, Dun B, Liu B, et al. A novel monoclonal antibody associated with glucoside kills gastric adenocarcinoma AGS cells based on glycosylation target[J]. Journal of Cellular and Molecular Medicine, 2022, 26(18): 4781-4791.(IF 5.295)

[4] Zhou H, Wan S, Luo Y, et al. Hepatitis B virus X protein induces ALDH2 ubiquitin-dependent degradation to enhance alcoholic steatohepatitis[J]. Gastroenterology Report, 2023, 11: goad006.(IF 3.6)

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