①若吸光值大于3.0,请确认比色皿是否为石英比色皿(Q);
②若A2测定大于1.5或∆A大于1.0,建议将粗酶液使用蒸馏水适当稀释后再进行测定;若∆A小于0.02,建议适当增加样本量或延长酶促反应时间(延长5-10 min)后再进行测定,计算时相应修改;
③准确在30 s和90 s处完成吸光值测定,以保证实验结果的准确性和重复性;
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