①粗酶液制备过程必须在冰上进行,保持低温环境以防止酶变性失活;
②试验过程中试剂二、试剂三和粗酶液需置于冰上放置,以防止变性和失活;
③若ΔA>0.5(96孔UV板>0.3)时,建议将粗酶液适当稀释后再进行测定,计算时相应修改;
④准确在10 s和70 s处完成测定,以确保实验结果的准确性和重复性;若使用96孔UV板进行测定,应使用多道移液器且分批进行检测,以确保组间反应时间一致;
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