NAD Kinase (NADK) Activity Assay Kit
NAD激酶(NADK)活性检测试剂盒
货号:AKCO008M
规格: 100T/48S
检测设备:酶标仪
可检测样本数:48 Samples
In Stock
BOXBIO提供专业的指标检测服务,如需检测服务,请点击右侧按钮
检测服务
Product Information
NAD Kinase (NADK) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:7 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5)
Detection Principle
NAD激酶(NADK)能够催化NAD+磷酸化,生成NADP+,NADP+进一步被6-磷酸葡萄糖脱氢酶还原为NADPH,NADPH通过PMS的递氢作用还原氧化型噻唑蓝(MTT),通过测定570 nm处吸光值变化即可表征NAD激酶的活性。
  • 检测方法: MTT法
  • 检测波长: 570 nm
  • 信号响应: 递增型
Notices

①提取过程必须在冰上完成,以防止酶变性失活;

②反应过程中试剂和粗酶液应置于冰上放置,以免变性和失活;

③若测量样本数量过多,可以将试剂二、五、六、七按比例配成工作液使用;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Chai J, Li J, Liu Q, et al. Differential changes in respiratory metabolism and energy status in the outer pericarp and core tissues affect the ripening of ‘Xuxiang’kiwifruit[J]. Postharvest Biology and Technology, 2024, 212: 112876. (IF 7.0)

[2] Li L X, Yu L Y, Wang B, et al. Impact of overexpressing NADH kinase on glucoamylase production in Aspergillus niger[J]. Journal of Industrial Microbiology and Biotechnology, 2022, 49(4): kuac015.(IF 4.258)

Related Products
Publication Reward
BOXBIO 助研基金申请
JOIN
*发表文章中注明Boxbio、北京盒子生工科技有限公司(Beijing Boxbio Science & Technology Co.,Ltd.) 即可申请。
Calculator
The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
  • x
    =
    x
Make Science Easy
Personal Assistant
BoxLab Provides Butler service
Professional Technical support
Product Innovation Protocol
Quality Control
Box QC&AS Provides
Strict Quality Management Process
Considerate After Sales service
Professional Service
Box Customer Service Provides
Pre-sales Service
Product ordering and cooperation
北京盒子生工科技有限公司旗下产品
版权所有:Copyright(C)2020 北京盒子生工科技有限公司