Thioredoxin Reductase (TrxR) Activity Assay Kit
硫氧还蛋白还原酶活性检测试剂盒
货号:AKCO010C
规格: 50T/48S
检测设备:可见分光光度计
可检测样本数:48 Samples
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Product Information
Thioredoxin Reductase (TrxR) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:6 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
硫氧还蛋白还原酶(TrxR)催化NADPH还原DTNB生成TNB和NADP+,TNB在412 nm处具有特征吸收峰,通过测定TNB的增加速率即可表征硫氧还蛋白还原酶的活性。
  • 检测方法: DTNB法
  • 检测波长: 412 nm
  • 信号响应: 递增型
Notices

①组织及血液样品一般需要使用蒸馏水适当稀释后再进行测定(5-10倍);

②试剂一中含有约0.1 mg/mL的蛋白,测定样品蛋白浓度时需要减去试剂一自身的蛋白含量;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhong Z, Li H, Li Z, et al. Inhibiting thioredoxin glutathione reductase is a promising approach to controlling Cryptocaryon irritans infection in fish[J]. Veterinary Parasitology, 2023: 109972.(IF 2.6)

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