Glucose 6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit
6-磷酸葡萄糖脱氢酶活性检测试剂盒
货号:AKCO013M
规格: 110T/100S
检测设备:酶标仪
可检测样本数:100 Samples
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Product Information
Glucose 6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:100 Samples
  • 主要检测设备及配套:酶标仪/96孔UV板
  • 预计测定时间:5 h (100 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
6-磷酸葡萄糖脱氢酶可催化6-磷酸葡萄糖氧化为6-磷酸葡萄糖酸内酯,同时将NADP还原为NADPH,NADPH在340 nm处具有特征吸收峰,通过吸光值的增加速率即可表征6-磷酸葡萄糖脱氢酶的活性。
  • 检测方法: NADPH速率法
  • 检测波长: 340 nm
  • 信号响应: 递增型
Notices

①测定过程中粗酶液应保持冰上放置,以免变性和失活;

②若ΔA大于0.5(96孔UV板大于0.3),建议将粗酶液使用提取液适当稀释;若ΔA小于0.02,建议适当增加样本量或延长反应时间后再进行测定,计算时相应修改;

③准确在相应时间点完成吸光值测定,以保证实验结果的准确性;若使用96孔UV板测定时,应使用多道移液器且分批进行测定,以确保组间反应时间一致;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Shen Y, Wang L, Ji B, et al. Stimulus-responsive nanomedicine mediated by metabolic intervention mechanisms to amplify redox anticancer therapy[J]. Chemical Engineering Journal, 2024: 150130. (IF 15.1)

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