Glucose 6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit
6-磷酸葡萄糖脱氢酶活性检测试剂盒
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①测定过程中粗酶液应保持冰上放置,以免变性和失活;
②若ΔA大于0.5,建议将粗酶液使用提取液适当稀释后再进行测定;若ΔA小于0.02,建议适当增加样本量或延长反应时间后再进行测定,计算时相应修改;
③准确在相应时间点完成吸光值测定,以保证实验结果的准确性和重复性;
[1] Shen Y, Wang L, Ji B, et al. Stimulus-responsive nanomedicine mediated by metabolic intervention mechanisms to amplify redox anticancer therapy[J]. Chemical Engineering Journal, 2024: 150130. (IF 15.1)