NAD-Malic Enzyme (NAD-ME) Activity Assay Kit
NAD-苹果酸酶活性检测试剂盒
货号:AKCO016U
规格: 50T/48S
检测设备:紫外分光光度计
可检测样本数:48 Samples
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Product Information
NAD-Malic Enzyme (NAD-ME) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:48 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:6 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
NAD-苹果酸酶能够催化苹果酸和NAD+生成丙酮酸和NADH,NADH在340 nm处具有特征吸收峰,通过吸光值变化速率即可表征NAD-苹果酸酶的活性。
  • 检测方法: NADH速率法
  • 检测波长: 340 nm
  • 信号响应: 递增型
Notices

①提取液中含有约0.1 mg/mL的蛋白,测定样品蛋白浓度时需要减去试剂一自身的蛋白含量;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
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