Coenzyme Ⅱ NADP (H) Content Assay Kit
辅酶Ⅱ NADP(H)含量检测试剂盒
货号:AKCO018C
规格: 120T/25S
检测设备:可见分光光度计
可检测样本数:25 Samples
In Stock
BOXBIO提供专业的指标检测服务,如需检测服务,请点击右侧按钮
检测服务
Product Information
Coenzyme Ⅱ NADP (H) Content Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:25 Samples
  • 主要检测设备及配套:可见分光光度计/1 mL玻璃比色皿(d=10 mm)
  • 预计测定时间:4 h (25 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
    无水乙醇(C2H6O,MW=46.07,CAS: 64-17-5)
Detection Principle
通过酸性和碱性提取液分别提取样品中NADP+和NADPH,NADPH可通过PMS的递氢作用,还原氧化型噻唑蓝(MTT)生成甲瓒,产物在570 nm处具有特征吸收峰;NADP+可被6-磷酸葡萄糖脱氢酶(Glucose 6-Phosphate Dehydrogenase, G6PDH)还原为NADPH,进一步采用MTT还原法生成甲瓒,通过吸光值变化即可定量检测辅酶Ⅱ NADP (H) 的含量。
  • 检测方法: MTT还原法
  • 检测波长: 570 nm
  • 信号响应: 递增型
Refreence Information
  • 标准物质: NADP+ & NADH
  • 参考标准: y=0.6226x-0.0099 (R2=0.999)
  • 标准线性范围: 0.08-2.0 nmol/mL
  • 检测限: 0.02 nmol/mL
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices

①试剂二、试剂三、试剂四配制后有效期较短,为便于试验安排,各附赠一瓶作为备用,每瓶均可满足至少25个样本的测定;

②2 µmol/mL NADP+和2 µmol/mL NADPH标准液配制后4℃可保存1周,严禁-20℃保存;

③实验过程中吸取上清液时应避免吸入沉淀物而影响结果的准确性;

④反应时间对实验结果有较大影响建议精确控制,测定样本较多时建议分批进行测定,以避免组内反应时间不一致对结果造成影响;

⑤反应体系中试剂不能按比例配制为混合液使用,必须按反应体系顺序依次加入;

⑥若A测定大于1.2,建议将待测样本适当稀释后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Cui Z, Zhong Y, Sun Z, et al. Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica[J]. Nature Communications, 2023, 14(1): 8480.(IF 16.6)

[2] Fang L, Chen Y, He Q, et al. Mining novel gene targets for improving tolerance to furfural and acetic acid in Yarrowia lipolytica using whole-genome clustered regularly interspaced short palindromic repeats interference library[J]. Bioresource Technology, 2024: 130764. (IF 11.4)

[3] Li L X, Yu L Y, Wang B, et al. Impact of overexpressing NADH kinase on glucoamylase production in Aspergillus niger[J]. Journal of Industrial Microbiology and Biotechnology, 2022, 49(4): kuac015.(IF 4.258)

[4] Chen Y, Xin Q, Pan L, et al. Improved Recombinant Expression of Maltogenic α-Amylase AmyM in Bacillus subtilis by Optimizing Its Secretion and NADPH Production[J]. Fermentation, 2023, 9(5): 475.(IF 3.7)

[5] Zhang B, Li Y, Liu N, et al. AP39, a novel mitochondria-targeted hydrogen sulfide donor ameliorates doxorubicin-induced cardiotoxicity by regulating the AMPK/UCP2 pathway[J]. Plos one, 2024, 19(4): e0300261. (IF 3.7)

Related Products
Publication Reward
BOXBIO 助研基金申请
JOIN
*发表文章中注明Boxbio、北京盒子生工科技有限公司(Beijing Boxbio Science & Technology Co.,Ltd.) 即可申请。
Calculator
The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
  • x
    =
    x
Make Science Easy
Personal Assistant
BoxLab Provides Butler service
Professional Technical support
Product Innovation Protocol
Quality Control
Box QC&AS Provides
Strict Quality Management Process
Considerate After Sales service
Professional Service
Box Customer Service Provides
Pre-sales Service
Product ordering and cooperation
北京盒子生工科技有限公司旗下产品
版权所有:Copyright(C)2020 北京盒子生工科技有限公司