①试剂二、试剂三、试剂四配制后有效期较短,为便于试验安排,各附赠一瓶作为备用,每瓶均可满足至少25个样本的测定;
②2 µmol/mL NADP+和2 µmol/mL NADPH标准液配制后4℃可保存1周,严禁-20℃保存;
③实验过程中吸取上清液时应避免吸入沉淀物而影响结果的准确性;
④反应时间对实验结果有较大影响建议精确控制,测定样本较多时建议分批进行测定,以避免组内反应时间不一致对结果造成影响;
⑤反应体系中试剂不能按比例配制为混合液使用,必须按反应体系顺序依次加入;
⑥若A测定大于1.2,建议将待测样本适当稀释后再进行测定,计算时相应修改;
[1] Cui Z, Zhong Y, Sun Z, et al. Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica[J]. Nature Communications, 2023, 14(1): 8480.(IF 16.6)
[2] Fang L, Chen Y, He Q, et al. Mining novel gene targets for improving tolerance to furfural and acetic acid in Yarrowia lipolytica using whole-genome clustered regularly interspaced short palindromic repeats interference library[J]. Bioresource Technology, 2024: 130764. (IF 11.4)
[3] Li L X, Yu L Y, Wang B, et al. Impact of overexpressing NADH kinase on glucoamylase production in Aspergillus niger[J]. Journal of Industrial Microbiology and Biotechnology, 2022, 49(4): kuac015.(IF 4.258)
[4] Chen Y, Xin Q, Pan L, et al. Improved Recombinant Expression of Maltogenic α-Amylase AmyM in Bacillus subtilis by Optimizing Its Secretion and NADPH Production[J]. Fermentation, 2023, 9(5): 475.(IF 3.7)
[5] Zhang B, Li Y, Liu N, et al. AP39, a novel mitochondria-targeted hydrogen sulfide donor ameliorates doxorubicin-induced cardiotoxicity by regulating the AMPK/UCP2 pathway[J]. Plos one, 2024, 19(4): e0300261. (IF 3.7)