①试剂二、试剂三、试剂四配制后有效期较短,为便于试验安排,各附赠一瓶作为备用,每瓶均可满足至少50个样本的测定;
②2 µmol/mL NADP+和2 µmol/mL NADPH标准液配制后4℃可保存1周,严禁-20℃保存;
③实验过程中吸取上清液时应避免吸入沉淀物而影响结果的准确性;
④反应时间对实验结果有较大影响建议精确控制,测定样本较多时建议分批进行测定,以避免组内反应时间不一致对结果造成影响;
⑤反应体系中试剂不能按比例配制为混合液使用,必须按反应体系顺序依次加入;
⑥若A测定大于0.8(96孔板)或1.2(微量玻璃比色皿),建议将待测样本适当稀释后再进行测定,计算时相应修改;
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