Nitrate Reductase (NR) Activity Assay Kit
硝酸还原酶活性检测试剂盒
货号:AKNM001U
规格: 60T/50S
检测设备:紫外分光光度计
可检测样本数:50 Samples
In Stock
BOXBIO提供专业的指标检测服务,如需检测服务,请点击右侧按钮
检测服务
Product Information
Nitrate Reductase (NR) Activity Assay Kit
-20℃ Dry Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:紫外分光光度计/1 mL 石英比色皿(d=10 mm)
  • 预计测定时间:5 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
硝酸还原酶可催化硝酸盐还原为亚硝酸盐,NO3-+NADH+H+→ NO2-+NAD++H2O,NADH在340 nm处具有特征吸收峰,通过吸光值变化即可表征硝酸还原酶的活性。
  • 检测方法: NADH速率法
  • 检测波长: 340 nm
  • 信号响应: 递减型
Notices

①硝酸还原酶易失活,粗酶液制备过程需在冰上迅速完成;

②空白组为各试剂组分质量的检测孔,正常情况下变化不超过0.05;

③准确在规定时间点完成吸光值的测定,以确保实验结果的准确性和重复性;

④试剂三有效期较短,为便于试验安排附赠1瓶作为备用,每瓶均可满足至少50个样本的测定;

⑤若A测定大于1.2或者∆A大于0.5时,建议将粗酶液适当稀释后再进行测定;若∆A测定小于0.01,可适当延长酶促反应时间后再进行测定,计算时相应修改;

注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhou L, Zhao B, Zhuang W Q. Double-edged sword effects of dissimilatory nitrate reduction to ammonium (DNRA) bacteria on anammox bacteria performance in an MBR reactor[J]. Water Research, 2023, 233: 119754.(IF 12.8)

[2] Zhou P, Jiang Y, Adeel M, et al. Nickel Oxide Nanoparticles Improve Soybean Yield and Enhance Nitrogen Assimilation[J]. Environmental Science & Technology, 2023, 57(19): 7547-7558.(IF 11.4)

[3] Yang L, Han R, Duan Y, et al. Exogenous application of silicon and selenium improves the tolerance of tomato plants to calcium nitrate stress[J]. Plant Physiology and Biochemistry, 2024: 108416. (IF 6.5)

[4] Guo Y, Li H, Hao Y, et al. Size Effects of Copper Oxide Nanoparticles on Boosting Soybean Growth via Differentially Modulating Nitrogen Assimilation[J]. Nanomaterials, 2024, 14(9): 746. (IF 5.3)

[5] An J, Sun L, Liu M, et al. Influences of Growth Stage and Ensiling Time on Fermentation Characteristics, Nitrite, and Bacterial Communities during Ensiling of Alfalfa[J]. Plants, 2023, 13(1): 84.(IF 4.5)

[6] Jia Z, Zhang J, Jiang W, et al. Different nitrogen concentrations affect strawberry seedlings nitrogen form preferences through nitrogen assimilation and metabolic pathways[J]. Scientia Horticulturae, 2024, 332: 113236. (IF 4.3)

[7] Zhu X, Lin F, Sun J, et al. Effects of Weak Electric Fields on the Denitrification Performance of Pseudomonas stutzeri: Insights into Enzymes and Metabolic Pathways[J]. Microorganisms, 2024, 12(6): 1218. (IF 4.1)

Related Products
Publication Reward
BOXBIO 助研基金申请
JOIN
*发表文章中注明Boxbio、北京盒子生工科技有限公司(Beijing Boxbio Science & Technology Co.,Ltd.) 即可申请。
Calculator
The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
  • x
    =
    x
Make Science Easy
Personal Assistant
BoxLab Provides Butler service
Professional Technical support
Product Innovation Protocol
Quality Control
Box QC&AS Provides
Strict Quality Management Process
Considerate After Sales service
Professional Service
Box Customer Service Provides
Pre-sales Service
Product ordering and cooperation
北京盒子生工科技有限公司旗下产品
版权所有:Copyright(C)2020 北京盒子生工科技有限公司