Nitrite Reductase (NiR) Activity Assay Kit
亚硝酸还原酶活性检测试剂盒
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Product Information
Nitrite Reductase (NiR) Activity Assay Kit
4℃ Wet Ice Transportation
- 检测样本量：50 Samples
- 主要检测设备及配套：酶标仪/96孔板
- 预计测定时间：4 h (50 Samples)
- 试剂储存条件：按照标签要求储存
-
需自备试剂:无
Detection Principle
亚硝酸还原酶能够将NO2-还原为NO，使样品中参与重氮化反应生成紫红色化合物的NO2-减少，重氮化反应产物在540 nm处具有特征吸收峰，通过吸光值变化即可表征亚硝酸还原酶的活性。
- 检测方法： 萘胺比色法
-
检测波长： 540 nm
-
信号响应： 递减型
Refreence Information
- 标准物质： Sodium Nitrite
- 参考标准： y=2.3395x+0.0109 (R2=0.9999)
- 标准线性范围： 0.025-0.5 μmol/mL
- 检测限： -
- 注：不同仪器及比色材质会对结果产生影响，以实际测定值为准。
Notices
①若∆A测定超出标准线性吸光值范围：高于最高值建议适当增加样本量后再进行测定，低于最低值建议将粗酶液适当稀释后再进行测定，计算时相应修改；
注： 为保证结果准确且避免试剂损失，测定前请仔细阅读说明书（以实际收到说明书为准），确认试剂储存和准备是否充分，操作步骤是否清楚，且务必取2-3个预期差异交的样本进行预测定，过程中问题请您及时与工作人员联系。
Product Citation
[1] Zhou L, Zhao B, Zhuang W Q. Double-edged sword effects of dissimilatory nitrate reduction to ammonium (DNRA) bacteria on anammox bacteria performance in an MBR reactor[J]. Water Research, 2023, 233: 119754.(IF 12.8)
[2] Yang L, Han R, Duan Y, et al. Exogenous application of silicon and selenium improves the tolerance of tomato plants to calcium nitrate stress[J]. Plant Physiology and Biochemistry, 2024: 108416. (IF 6.5)
[3] An J, Sun L, Liu M, et al. Influences of Growth Stage and Ensiling Time on Fermentation Characteristics, Nitrite, and Bacterial Communities during Ensiling of Alfalfa[J]. Plants, 2023, 13(1): 84.(IF 4.5)
[4] Jia Z, Zhang J, Jiang W, et al. Different nitrogen concentrations affect strawberry seedlings nitrogen form preferences through nitrogen assimilation and metabolic pathways[J]. Scientia Horticulturae, 2024, 332: 113236. (IF 4.3)
[5] Zhu X, Lin F, Sun J, et al. Effects of Weak Electric Fields on the Denitrification Performance of Pseudomonas stutzeri: Insights into Enzymes and Metabolic Pathways[J]. Microorganisms, 2024, 12(6): 1218. (IF 4.1)
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Calculator
The Dilution Calculator Equation
Concentration (start)xVolume (start)= Concentration (final)× Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
This equation is commonly abbreviated as: C1V1 = C2V2
Sample(S):可测定的样品数量；
★根据此数量计算需要的试剂盒个数。
Size
120T/50S
Test(T):总反应次数；
★每个试剂盒均预留了至少5个（分光光度法）和10个（微板法）样本预实验的试剂用量；
★若需要制作标准曲线则至少能够满足2次制作标准曲线的试剂用量（15个）。
