Nitrite Reductase (NiR) Activity Assay Kit
亚硝酸还原酶活性检测试剂盒
货号:AKNM004M-50S
规格: 120T/50S
检测设备:酶标仪
可检测样本数:50 Samples
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Product Information
Nitrite Reductase (NiR) Activity Assay Kit
4℃ Wet Ice Transportation
  • 检测样本量:50 Samples
  • 主要检测设备及配套:酶标仪/96孔板
  • 预计测定时间:4 h (50 Samples)
  • 试剂储存条件:按照标签要求储存
  • 需自备试剂:
Detection Principle
亚硝酸还原酶能够将NO2-还原为NO,使样品中参与重氮化反应生成紫红色化合物的NO2-减少,重氮化反应产物在540 nm处具有特征吸收峰,通过吸光值变化即可表征亚硝酸还原酶的活性。
  • 检测方法: 萘胺比色法
  • 检测波长: 540 nm
  • 信号响应: 递减型
Refreence Information
  • 标准物质: Sodium Nitrite
  • 参考标准: y=2.3395x+0.0109 (R2=0.9999)
  • 标准线性范围: 0.025-0.5 μmol/mL
  • 检测限: -
  • 注:不同仪器及比色材质会对结果产生影响,以实际测定值为准。
Notices
①若∆A测定超出标准线性吸光值范围:高于最高值建议适当增加样本量后再进行测定,低于最低值建议将粗酶液适当稀释后再进行测定,计算时相应修改;
注: 为保证结果准确且避免试剂损失,测定前请仔细阅读说明书(以实际收到说明书为准),确认试剂储存和准备是否充分,操作步骤是否清楚,且务必取2-3个预期差异交的样本进行预测定,过程中问题请您及时与工作人员联系。
Product Citation

[1] Zhou L, Zhao B, Zhuang W Q. Double-edged sword effects of dissimilatory nitrate reduction to ammonium (DNRA) bacteria on anammox bacteria performance in an MBR reactor[J]. Water Research, 2023, 233: 119754.(IF 12.8)

[2] Yang L, Han R, Duan Y, et al. Exogenous application of silicon and selenium improves the tolerance of tomato plants to calcium nitrate stress[J]. Plant Physiology and Biochemistry, 2024: 108416. (IF 6.5)

[3] An J, Sun L, Liu M, et al. Influences of Growth Stage and Ensiling Time on Fermentation Characteristics, Nitrite, and Bacterial Communities during Ensiling of Alfalfa[J]. Plants, 2023, 13(1): 84.(IF 4.5)

[4] Jia Z, Zhang J, Jiang W, et al. Different nitrogen concentrations affect strawberry seedlings nitrogen form preferences through nitrogen assimilation and metabolic pathways[J]. Scientia Horticulturae, 2024, 332: 113236. (IF 4.3)

[5] Zhu X, Lin F, Sun J, et al. Effects of Weak Electric Fields on the Denitrification Performance of Pseudomonas stutzeri: Insights into Enzymes and Metabolic Pathways[J]. Microorganisms, 2024, 12(6): 1218. (IF 4.1)

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