①所有相关器皿耗材都应为RNase-free产品,操作过程要小心,带口罩、手套避免环境中RNA酶污染样品;
②RNA在水溶液中OD值可能在1.5-1.9之间,但这并不表示RNA不纯,需电泳检测;③组织若不经过处理,RNA易被降解,新鲜组织应及时放入液氮中速冻并立即储存于-70℃,冻存的组织不能反复冻融以避免RNA的降解,样品也可以在匀浆后加入裂解液,储存于-70℃;[1] Guo D, Li J, Liu P, et al. The jasmonate pathway promotes nodule symbiosis and suppresses host plant defense in Medicago truncatula[J]. Molecular Plant, 2024. (IF 17.1)
[2] Feng Y, Huang Z, Lu F, et al. 8-Br-cGMP activates HSPB6 and increases the antineoplastic activity of quinidine in prostate cancer[J]. Cell Death Discovery, 2024, 10(1): 90. (IF 7.0)
[3] Huang L, Jia X, Zhao Y, et al. Effects of elevated CO2 on phenolics in black locust seedlings inoculated with arbuscular mycorrhizal fungi under cadmium pollution by 13C isotopic tracer technique[J]. Plant and Soil, 2023: 1-20.(IF 4.9)
[4] Zhao S Y, Zhao H H, Wang B H, et al. Rhein alleviates advanced glycation end products (AGEs)-induced inflammatory injury of diabetic cardiomyopathy in vitro and in vivo models[J]. Journal of Natural Medicines, 2023, 77(4): 898-915.(IF 3.3)